Blood Glucose Sampling (BM)

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Equipment

Equipment you will require for blood glucose analysis:

BM device

  • Blood glucose (BM) meter
  • Test strips for the meter (see red arrow)
  • Spring-loaded device for finger-pricking
  • Sterile (alcohol) swab
  • Gloves (non-sterile)
  • Cotton wool ball/swabs
  • Watch


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    The Procedure


    Useful fact: The test is usually called a 'BM' test after 'Boehringer Mannheim', a German pharmaceutical company (now named 'Roche').

    Protocol:

    1. Collect your equipment, introduce yourself to the patient, confirm their identity, explain what you are about to do, and obtain consent.

    2. Ensure the machine is calibrated for use. This will probably be done for you in an exam (or on the wards, ask the nurses to show you!), but make sure that you have checked/mentioned that this is done. Put on your gloves.

    3. Make sure the patient has also washed their hands (or clean the sampling area with the alcohol swab, as this will reduce false readings.

    Fingerprick blood

    4. Using the finger-pricking device, prick the patient's finger at the side of the fingertip. Try not to choose a finger that has been tested recently.

    5. Squeeze the tip of the chosen finger to get some blood accumulating at the tip. Hold the test strip by the tip and get a large drop of blood onto the sample area of the test strip. Once you have enough to cover the sample area, place a cotton wool ball onto the skin and get the patient to apply pressure to prevent further bleeding.

    How it all works...

    6. Insert the test strip into the machine. Different machines have different techniques by which they operate...be sure to practice on the ones in use at your hospital.

    7. Press the 'analyse' button and wait for the machine to analyse the blood. This can sometimes take a while to complete. Record the result.

    8. Dispose of the lancet and test strip into the sharps bin. Thank the patient, and clear up. Wash your hands.


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    Oral Glucose Tolerance Test (OGTT)


    1. This test is usually performed to confirm more 'borderline' cases of Diabetes Mellitus (either those in the category of 'impaired fasting glucose' levels or when 2 indeterminate fasting glucose results are obtained).

    2. It needs to be performed on a fasting patient. The easiest way is for the patient to fast overnight. The 'loading dose' of glucose is given as 75g of glucose in 250-350 ml water (taken orally).

    3. Blood glucose levels are then measured by samples taken in the fasting state (pre-glucose load) and at two hours after the glucose has been ingested.

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    Results & Interpretation

    BM results need to be confirmed by formal venous (plasma) measurements before any diagnosis of Diabetes can be made. The results of these investigations can be interpreted as follows (please note that these criteria change all the time, so it is probably best to check that newer guidelines have not been introduced since this page was last updated):

    Values:

    Fasting glucose 2-hour glucose Interpretation
    >7.0 >11.1 Diabetes mellitus
    >6.1 & <7.0 >7.8 & <11.1 Impaired glucose tolerance
    >6.1 & <7.0 <7.8 Impaired fasting glucose tolerance
    <6.0 Normal

    Diabetes Mellitus (DM) diagnosis:

    (If symptomatic), DM is confirmed by:

    1. Random plasma glucose of >11.1mmol/l
    2. Fasting plasma glucose >7.0mmol/l
    3. OGTT 2-hour value of >11.1mmol/l (on follow up of impaired fasting glucose)

    Note: Blood glucose is 10% less than that of plasma glucose.

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